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Antibody Target
Bad
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Target Alternative Name
BAD antibody,BAD human antibody,BBC 2 antibody,BBC2 antibody,BBC6 antibody,Bcl 2 Antagonist of Cell Death antibody,Bcl 2 Binding Component 6 antibody,BCL X / BCL 2 Binding Protein antibody,BCL X Binding Protein antibody,Bcl XL/Bcl 2 Associated Death Promoter antibody,Bcl-2-binding component 6 antibody,Bcl-2-like protein 8 antibody,Bcl-XL/Bcl-2-associated death promoter antibody,Bcl2 antagonist of cell death antibody,BCL2 antagonist of cell death protein antibody,BCL2 associated agonist of cell death antibody,Bcl2 Associated Death Promoter antibody,BCL2 binding component 6 antibody,BCL2 binding protein antibody,Bcl2 Like 8 Protein antibody,Bcl2-L-8 antibody,BCL2L8 antibody,BclXL antibody,Proapoptotic BH3 Only Protein antibody.
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Antibody Type
Monoclonal
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Species Reactivities
Human, Mouse, Rat
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Immunogen Sequence
Full length human Bad
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Accession
Q92934
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Gene Id
572
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.2 mg/ml
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Formulation
100 µg (200 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
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Purification
Affinity purified rabbit IgG
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Application
Western blot
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Positive Control
Western Blot
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Application And Usages
Western blot: 1-4 µg
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Description
Bad is a member of the Bcl-2 family proteins. Within the Bcl-2 homology domains 1 and 2 (BH1 and BH2), Bad shows significant homology to Bcl-2 and Bcl-x. Bcl-2 is known to block several apoptosis signals and is considered to be a central downstream cell death repressor. Bcl-XL represses apoptosis, but its short form, Bcl-XS, promotes cell death. Bax is known to homodimerize as well as heterodimerize with Bcl-2. An excess concentration of Bax opposes the ability of Bcl-2 to repress cell death. Bad can selectively dimerize with Bcl-xL and Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1, or itself. In mammalian cells, Bad binds more strongly to Bcl-xL than Bcl-2, which may explain why Bad reverse the death repressor activity of Bcl-xL, but not that of Bcl-2. The formation of the Bad-Bcl-xL heterodimer displaces Bax and restores favorable conditions for apoptosis.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
