PDGF-BB Recombinant Protein

• Catalog number
  40-607
• Price
  Please ask
• Size
  0.01 mg

• About recombinant protein
  PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-β. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids). .
• Other name of the recombinant
  SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
• Recombinant in
  E. coli
• His tag on the recombinant protein
  no His tag
• SEQUENCE
  SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
• Activity
  Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/mL.
• Keep at
  The lyophilized PDGF-BB recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted PDGF-BB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing PDGF-BB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  4505681
• Accession nr
  NP_002599.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  5155
• Gene name
  platelet-derived growth factor beta polypeptide
• Source
  Homo sapiens
• Uniprot
  P01127
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PDGF-BB   Protein  
• Gene symbol
  PDGFB
• Short name
  PDGF-BB Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  PDGF-BB Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8800
• Gene
  PDGFB
• Long gene name
  platelet derived growth factor subunit B
• Synonyms gene
  • SIS
• Synonyms gene name
  • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
  • platelet-derived growth factor beta polypeptide
• Synonyms
  • SSV
• Synonyms name
  • oncogene SIS
  • becaplermin
• Locus
  22q13.1
• Discovery year
  2001-06-22
• Entrez gene record
  5155
• Pubmed identfication
  • 2991848
  • 1661670
• RefSeq identity
  • NM_002608
• VEGA ID
  OTTHUMG00000151029

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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