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Gene info
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32- kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc.. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17- kDa and a small subunit of 12- kDa. Caspase 3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase 3 proteolytically cleaves and activates other proteins. Caspase 3 has been associated with apoptosis.
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Gene names
CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama
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Antibodie s label
unlabelled serum
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Purity
Protein G Column
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
[31A1067 ]
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Immunoglobulin
IgG
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Spicific to
The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
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Antigen
Full-length recombinant human caspase-3 protein was used as immunogen.
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Immuno application
Western Blot, immuno-histo-chemistry
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Antibody advice
Caspase-3 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 ug/mL.
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Suspention pH azide
PBS, 0.05% BSA, 0.05% sodium azide.
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Keep at
Store Caspase-3 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Human, Mouse
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Omim nr
77416852
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Ncbi nr
P42574
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Vial with antibody
in solution
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NCBI GENE nr
836
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GENE symbol
CASP3
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Antigene name
caspase 3, apoptosis-related cysteine peptidase
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Source
Homo sapiens
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Protein nr
P42574
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
