Target of this antibody:
Alternative names of antibody target:
H4FB , HIST1H4B , HIST1H4F, H4FN, H4FH
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide corresponding to the N-terminus of human Histone H4
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Histone H4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml), in immunoprecipitation (20-40 µg/ml) and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~11 kDa histone H4 protein. It does not cross-react with other histones.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.