Enterokinase, Light Chain, human recombinant

  • Catalog number
    7529-500
  • Price
    Please ask
  • Size
    500 ug
  • Synonyms
    Enteropeptidase, Serine protease 7, Transmembrane protease serine 15
  • Alternative_names
    Enteropeptidase, Serine protease 7, Transmembrane protease serine 15
  • Description
    Activates trypsinogen to trypsin.
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Biological activity
    BioVision’s Enterokinase, Light Chain, has been tested for its ability to cleave a fluorogenic substrate Ac-DDDDK-AFC (Catalog # K758-100).
  • Results
    >12,000 mU/mg
  • Unit Definition
    1 U = Cleavage of 1 µmole per min of Ac-DDDDK-AFC at 37°C in 50 mM Tris, 1 mM CaCl2, 0.1% Tween 20, pH 8.0
  • Molecular Weight
    26.3 kDa
  • Storage Temp
    -80°C
  • Shipping
    Dry Ice
  • Shelf Life
    12 months
  • Concentration
    0.5 mg/ml
  • Appearance
    Liquid
  • Physical form description
    0.5 mg/ml in 25 mM Tris, 50 mM NaCl (pH 8.0) containing 50% glycerol
  • Background Information
    Enterokinase (EC 3.4.21.9) is a serine protease involved in the activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    Enterokinase, Light Chain, recombinant
  • Technique
    Recombinant, light, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    Enterokinase, Light epitope, H. sapiens Rec.
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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