Enterokinase, Light Chain, human recombinant
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Catalog number
7529-500
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Price
Please ask
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Size
500 ug
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Synonyms
Enteropeptidase, Serine protease 7, Transmembrane protease serine 15
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Alternative_names
Enteropeptidase, Serine protease 7, Transmembrane protease serine 15
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Description
Activates trypsinogen to trypsin.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Biological activity
BioVision’s Enterokinase, Light Chain, has been tested for its ability to cleave a fluorogenic substrate Ac-DDDDK-AFC (Catalog # K758-100).
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Results
>12,000 mU/mg
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Unit Definition
1 U = Cleavage of 1 µmole per min of Ac-DDDDK-AFC at 37°C in 50 mM Tris, 1 mM CaCl2, 0.1% Tween 20, pH 8.0
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Molecular Weight
26.3 kDa
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Storage Temp
-80°C
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Shipping
Dry Ice
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Shelf Life
12 months
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Concentration
0.5 mg/ml
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Appearance
Liquid
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Physical form description
0.5 mg/ml in 25 mM Tris, 50 mM NaCl (pH 8.0) containing 50% glycerol
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Background Information
Enterokinase (EC 3.4.21.9) is a serine protease involved in the activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Enterokinase, Light Chain, recombinant
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Technique
Recombinant, light, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Enterokinase, Light epitope, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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