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Available ordering format
Lyophilized
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Immunogen
Recombinant ClpC (C-terminal domain overexpressed as fusion with maltose-binding protein) Q55023
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP), immunohistochemistry (IHC)
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Connected products
AS08 344 | anti-ClpB1 ATP-dependent chaperone, rabbits antibodiesAS08 355 |anti-ClpB2 ATP-dependent chaperone, rabbit antibodiesAS9 459 | anti-ClpB-P | ClpB3, rabbit antibodiesAS13 2655 | anti-ClpP6 | chloroplast ClpP6 proteolytic subunit, rabbit antibodiesAS15 3077 | anti-pClpP | Chloroplastic ATP-dependent Clp protease proteolytic subunit 1, rabbit antibodiesAS07 253 | anti-HSP101 | ClpB heat shock protein, N-terminal, rabbit antibodiesAS08 287 | anti-HSP101 | ClpB heat shock protein, C-terminal, rabbit antibodiesAS08 355 | anti-slr0156 | ATP-dependent chaperone clpB, rabbit antibodiesAS08 344 | anti-slr1641 | ATP-dependent chaperone clpB, rabbit antibodies
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Recommended dilutions for use
1 : 5000 on 10 µg of total protein, detected with standard ECL (WB); 1: 1000 (IHC)
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Molecular weight expected аpparent
n.a. | 87 kDa
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Verified reactivity
Arabidopsis thaliana, Synechococcus sp., Chlamydomonas reinhardtii, Streptomyces sp.
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Possible reactivity
dicots including: Nicotiana tabacum, Pisum sativum, Solanum tuberosum, monocots including: Oryza sativa, Zea mays, trees. Populus trichocarpa, moss: Physcomitrella patens, red alga, Ostreococcus sp.
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No reactivity
different strains of Mycobacterium smegmatis
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Supplementary information
n.a.
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References
Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015. Rosano et al. (2011). Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. J Biol Chem. Aug 26;286(34):29671-80. (Western blot, Arabidopsis thaliana)Karradt et al. (2008) NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease. J Biol Chem 283: 32394-32403.Porankiewicz & Clarke (1997) Induction of the heat shock protein ClpB affects cold acclimation in the cyanobacterium Synechococcs sp. strain PCC7942. J Bacteriol 179:5111-5117.
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Scientific context
ClpC is a chloroplastic protein of the Hsp100 family. It is believed to function as a housekeeping enzyme, both in its capacity as an independent molecular chaperone and as the regulatory component of the Clp protease.
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Notes
Anti-ClpC antibodies will also recognized Arabidopsis thaliana isoform ClpC1 (At5g50920) and ClpC2 (At3g48870).
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Protein number
Q55023
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TAIR number
AT5G50920
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