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Also known as
Cytokeratin 5,8
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Other names
cytokeratin 8 polypeptide; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; K8; CK-8; keratin-8; cytokeratin-8; cytokeratin endo A; type-II keratin Kb8; keratin complex 2, basic, gene 8; keratin 8; Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
Krt8; Krt8; CYKER; Krt2-8; Krt2-8
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Gene name synonims
Krt8; Krt8; CYKER; Krt2-8; Krt2-8
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Other gene names
Krt8; Krt8; CYKER; Krt2-8; Krt2-8
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
5F173
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Bovine, Human (Homo sapiens), Mouse (Mus musculus), Porcine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human Cytokeratin 5,8. Species Crossreactivity: Bovine, murine, amphibia, porcine and hydra.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein A affinity chromatography.
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Form Appearance
Supplied as a liquid. Conjugated to FITC.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
