Human IL-2 ELISpotPLUS kit (HRP)

Categories

ELISpot Kits
Description

Pre-coated plates reduce assay time and minimize variability.
Enzyme Conjugate

NA
Format

pre-coated plates
Kit Detection Type

Streptavidin-HRP
Application

ELISpot
Reactivities

human
Analytes

IL2
Contents

Biotinylated detection mAb (MT8G10) ,Streptavidin-HRP,anti-CD3 mAb,Substrate (TMB),Pre-coated MSIP white plates (mAbs MT2A91/2C95)
Datasheet
Product Header

ELISpotPLUS for enumeration of cells secreting IL-2
GeneID

NA
Shelf Life

18 months
Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation

HRP Conjugated

Gene target

IL-2 ELISpotPLUS kit
Gene symbol

IL2, IL36G, IL25, IL22, IL18, IL37, IL1RAPL2, IL6R, IL17C, IL17D
Short name

IL-2 ELISpotPLUS kit ( )
Label

HRP
Species

Human, Humans
Alternative name

H. sapiens Interleukin-2 ELISpotPLUS reagent (horseradish peroxidase)
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:5997
Gene

IL1RAPL2
Long gene name

interleukin 1 receptor accessory protein like 2
Synonyms
- IL-1R9
- TIGIRR-1
- IL1RAPL-2
- IL1R9
GenBank acession
- AF181285
Locus

Xq22.3
Discovery year

1999-11-19
Entrez gene record

26280
Pubmed identfication
- 10757639
RefSeq identity
- NM_017416
Classification
- TIR domain containing
- Immunoglobulin like domain containing
- Interleukin receptors
VEGA ID

OTTHUMG00000022141
Locus Specific Databases
- Global Variome shared LOVD

Identity

HGNC:6019
Gene

IL6R
Long gene name

interleukin 6 receptor
Synonyms
- CD126
- IL-6R
- IL-1Ra
- IL6RA
- gp80
Synonyms name
- membrane glycoprotein 80
- interleukin 6 receptor subunit alpha
GenBank acession
- X12830
Locus

1q21.3
Discovery year

2001-06-22
Entrez gene record

3570
Pubmed identfication
- 8428753
- 12633863
RefSeq identity
- NM_000565
Classification
- CD molecules
- Interleukin receptors
- Immunoglobulin like domain containing
VEGA ID

OTTHUMG00000036073

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data