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Clone
S23b-49
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Immunogen
Fusion protein amino acids 84-309 of rat Shank2
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Antibody s full description
Mouse Anti-Rat SHANK Monoclonal IgG1 Antibody, Clone: S23b-49: ATTO 488
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Cortactin binding protein 1 Antibody, Cortactin SH3 domain-binding protein Antibody, Cortactin-binding protein 1 Antibody, CortBP1 Antibody, CTTNBP1 Antibody, GKAP/SAPAP interacting protein Antibody, GKAP/SAPAP-interacting protein Antibody, KIAA1022 Antibody, Proline rich synapse associated protein 1 Antibody, Proline-rich synapse-associated protein 1 Antibody, ProSAP1 Antibody, SH3 and multiple ankyrin repeat domains protein 2 Antibody, SHAN2_RAT Antibody, SHANK Antibody, Shank2 Antibody, SPANK-3 Antibody
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Raised in
Mouse
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Antibody s target
SHANK
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Primary research fields
Neuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Scaffolds
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Brandname
None
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Antibodies applications
WB, IHC, IP, ICC/IF, AM
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s dilutions
WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~160kDa. Recognizes Shank1, 2 and 3.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-327 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Cell Projection , Growth Cone
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer’s disease (3).
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Bibliography
1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Kim J.H., et al. (2009) Exp Mol Med. 41(4): 236-242. 3. Gong Y., et al. (2009) Brain Res. 1292: 191-198.
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Released date
13-Apr-2010
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PubMed number
23268962
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Tested applications
Western Blot
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Tested reactivity
Mouse
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NCBI number
Refer to NCBI
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Gene number
171093
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Protein number
Q9QX74
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (SMC-327). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (SMC-327) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SHANK (pan) Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49 (SMC-327). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody (SMC-327) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SHANK (pan) Antibody [S23b-49] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-327) Mouse Anti-SHANK Antibody [S23b-49] used in Western Blot (WB) on Rat brain membrane lysate (SMC-327)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
