Deubiquitinase Activity Assay Kit (Fluorometric)

• Catalog number
  K485-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay
• Summary
  • Detection method- Fluorescence (Ex/Em 350/440 nm) • Species reactivity- Mammalian • Applications-DUB Activity Assay is highly sensitive with the detecting limit approximately 0.25 μU
• Detection Method
  Fluorescence (Ex/Em 350/440 nm)
• Species Reactivity
  Mammalian
• Applications
  • Measurement of DUB activity in various tissues/cell extracts • Determination of DUB activity associated with pathological conditions • Characterization of activity of purified DUB enzymes
• Sample Type
  • Purified recombinant protein • Cell and tissue lysate
• Features Benefits
  • Simple procedure; takes only ~40 min • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cell activity and viability is tightly regulated by controlling the production and degradation of the thousands of different proteins in the cell. The proteasome is responsible for the majority of cellular protein degradation; however, drugs targeting the proteasome can have side effects caused by the lack of specificity associated with inhibiting the proteasome itself. Altering the ubiquitination state of target proteins is thus appealing as an alternative approach. Modification of the ubiquitin-mediated proteasome pathway has been shown to be a valid mechanism for treating a variety of diseases, all of which involve dysregulation of cellular proteostasis. As such, it is imperative that these ubiquitination signals also be reversible. The enzymes responsible for cleavage, and hence removal of ubiquitin from ubiquitinated proteins, are known as de-ubiquitinating enzymes, or DUBs. They are proteases that hydrolyze the isopeptide bond between an ubiquitin moiety and a lysine residue on its target protein. By removing the ubiquitin molecule, the protein escapes the fate of proteasomal degradation and remains a viable factor in the cell. BioVision’s Deubiquitinase Activity Assay Kit provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate to detect activity as low as 0.25 µU with purified enzyme.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Deubiquitinase   Activity   Kit   Fluorometric  
• Short name
  Deubiquitinase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Deubiquitinase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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