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Canine Interleukin 4 IL4 ELISA Kit 96 well plate

Canine Interleukin 4 IL4 ELISA Kit 96 well plate is available 3 times from Bioma labs

EKU05330 | Canine Interleukin 4 (IL4) ELISA Kit, 96 well plate size: 96T | 988.76 USD

Catalog number EKU05330
Supplier bioma
Price 988.76 USD
Size 96T
1. Gene info
Identity 6014
Gene IL4
Long gene name interleukin 4
  • BSF1
  • IL-4
  • BCGF1
  • BCGF-1
  • MGC79402
Synonyms name
  • B_cell stimulatory factor 1
  • lymphocyte stimulatory factor 1
  • B cell growth factor 1
GenBank acession
  • M23442
Locus 5q31.1
Discovery year 1988-08-10
Entrez gene record 3565
Pubmed identfication
  • 3016727
RefSeq identity
  • NM_000589
  • Interleukins
Havana BLAST/BLAT OTTHUMG00000059724

EKU05330 | Canine Interleukin 4 (IL4) ELISA Kit, 96 well plate size: 10x96T | 7,514.36 USD


EKU05330 | Canine Interleukin 4 (IL4) ELISA Kit, 96 well plate size: 5x96T | 4,158.01 USD

Verified reactivity Canis familiaris; Canine (Dog)
Detection limits 15.62-1000 pg/mL
Sensitivity limit 5,9 pg/mL
Samples to be analyzed Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay duration 3 hours
Verified applications ELISA
Assay class Sandwich
ELISA detection Colorimetric
Protein number O77762
Alternate protein number Please refer to SwissProt
Gene number 403785
Gene name Interleukin 4
Alternate gene name BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
Estimated production time 7-11 business days
Shipping requirements Blue ice
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for 8 months
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Interleukin 4 (IL4). No significant cross-reactivity or interference between Interleukin 4 (IL4) and analogues was observed.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 4 (IL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Protocol Please see ELISA's datasheet, otherwise contact us
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area Cytokine;Immune molecule;
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes For research use only. Not for diagnostic procedures.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is used for dendritic and T cell therapy. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13. Recombinant, GMP rec. E. coli interleukin-4 for cell culture supplied by GENTAUR. Free samples on request.
Description A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Gene targetInterleukin IL4
Short name Interleukin 4 (IL4) ELISA Kit, 96
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species From Canis lupus or the domestic dog we know mostly the Beagle to be used in drug discovery experiments.
Alternative name canine Interleukin 4 (interleukin 4) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, interleukin 4, BCGF-1 and BCGF1 and BSF-1 and BSF1 and IL-4, IL4 and IDBG-42701 and ENSG00000113520 and 3565, growth factor activity, Extracellular, Il4 and IDBG-172024 and ENSMUSG00000000869 and 16189, IL4 and IDBG-644671 and ENSBTAG00000015957 and 280824
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Interleukin 4 (IL4) ELISA Kit, 96 | Technique alternative |
EU:+32-(0)1-658-90-45 US:+1-(408)780-0908 [email protected]
  • Interleukin
  • IL4
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