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Stock availability
In Stock
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Scientific context
Aha2 shares 45% sequence homology with Aha1, however, little is known about the functional protein. Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
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Protein target
AHA2
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Protein reactivity
Mouse
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Certificate of analysis
This product has been certified >90% pure using SDS - PAGE analysis.
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Protein description
Mouse Recombinant AHA2 Protein
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Other name
ATPase hydrogen-exporting ATPase 2 Protein
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Primary research area
Cancer, Heat Shock
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Category
Protein
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Brand name
none
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Origin
Recombinant
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NCBI number
NM_172391.3
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Gene number
268390
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Protein number
Q8N9S3
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Verified applications
WB, SDS-PAGE, Functional Assay
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Relevant bio activity
AHA2 Protein
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Protein expression model
E. coli
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Protein charasterics
See included datasheet.
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Peptide sequence
MGHHHHHHMAKWGQGDPRWIVEEREDGTNVNNWHWTERDATIWSKGKLRELLVGIAMENEAGRCEISELKQVEGEASCNSRKGKLIFFYEWNIKLAWKGTVKESGAKHKGLIEIPSLSEENEINDTEVNVSKKKGDGEILKDLMRTTGTAKVREALGEYLKALKTEFTTGMILPTKAVATQELTLQRKLNENKLQASPVALGVRIPTVALHLTELFDTTVEQLYSIFTVKELVQKFSKSPAVLEAERGGKFQMFDGNISGEYVELVTNRKIIMKWRCRNWPEEHYATVELNFVPAPGQTELQLDCKGVPVCKEENMKFCWQKQHFEEIKGLLELTAQNA
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Protein purification
Affinity Purified
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Purity pourcentage
>90% High purity
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Recommended buffer for storage
25mM Trif buffer pH7.2, 150mM NaCl, 0.1mM DTT, 50% glycerol
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Protein concentration
Lot/batch specific. See included datasheet.
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Protein specificity
~38 kDa
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Protein tag
His tag
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Supplementary useful information
Please see included datasheet or contact us
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Bibliography
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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Release date
1-Aug-2011
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PubMed number
Not added. Please refer to PubMed
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Tested applications
to be tested
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Tested species reactivity
to be tested
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Representative figure legend
SDS-PAGE of ~38kDa his-tagged yeast Aha2 protein (SPR-313). SDS-Page of mouse AHA2 Protein (SPR-313)
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Warnings
Non-hazardous materials
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Protein origin
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants