Mouse Recombinant AHA1 Protein

• Catalog number
  SPR-311B
• Price
  Please ask
• Size
  100 µg

• Stock availability
  In Stock
• Scientific context
  Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
• Protein target
  AHA1
• Protein reactivity
  Mouse
• Certificate of analysis
  This product has been certified >90% pure using SDS-PAGE analysis. 2uM SPR-311 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
• Protein description
  Mouse Recombinant AHA1 Protein
• Other name
  Aha1 Protein, SHSA1 Protein, HSPC322 Protein, p38 Protein
• Primary research area
  Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
• Category
  Protein
• Brand name
  none
• Origin
  Recombinant
• NCBI number
  NP_666148.1
• Gene number
  217737
• Protein number
  Q8BK64
• Verified applications
  WB, SDS-PAGE, Functional Assay
• Relevant bio activity
  AHA1 Protein
• Protein expression model
  E. coli
• Protein charasterics
  See included datasheet.
• Peptide sequence
  MGHHHHHHMAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTEKLKTLFLAVRVENEEGKCEVTEVNKLDGEASINNRKGKLIFFYEWTIKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEDGVKLLREAVGIYISTLKTEFTQGMILPTVNGESVDPVGQPALKTETCKAKSAPSKSQAKPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPAALEADRGGKFHMVDGNVTGEFTDLVPEKHIAMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF
• Protein purification
  Affinity Purified
• Purity pourcentage
  >90% High purity
• Recommended buffer for storage
  25mM Hepes buffer pH7.2, 100mM KoAc, 50% glycerol
• Protein concentration
  Lot/batch specific. See included datasheet.
• Protein specificity
  ~38 kDa
• Protein tag
  His tag
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Supplementary useful information
  Please see included datasheet or contact us
• Protein cell localization
  Cytoplasm
• Bibliography
  1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
• Release date
  1-Aug-2011
• PubMed number
  Not added. Please refer to PubMed
• Tested applications
  to be tested
• Tested species reactivity
  to be tested
• Representative figure
• Representative figure legend
  SDS-PAGE of ~38kDa his-tagged mouse Aha1 protein (SPR-311). SDS-Page of mouse AHA1 Protein (SPR-311)
• Warnings
  Non-hazardous materials
• Protein origin
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Other related products
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  AHA1   Protein  
• Gene symbol
  AHSA1, AHSA2P
• Short name
  Mouse Recombinant AHA1 Protein
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  Mouse Rec. AHA1 Protein
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:1189
• Gene
  AHSA1
• Long gene name
  activator of HSP90 ATPase activity 1
• Synonyms gene
  • C14orf3
• Synonyms gene name
  • chromosome 14 open reading frame 3
  • AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
• Synonyms
  • p38
  • Aha1
  • hAha1
• GenBank acession
  • AJ243310
• Locus
  14q24.3
• Discovery year
  1999-07-01
• Entrez gene record
  10598
• Pubmed identfication
  • 26796264
• RefSeq identity
  • NM_012111
• VEGA ID
  OTTHUMG00000171539

Gene info

• Identity
  HGNC:20437
• Gene
  AHSA2P
• Long gene name
  activator of HSP90 ATPase homolog 2, pseudogene
• Synonyms gene
  • AHSA2
• Synonyms gene name
  • AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast)
  • activator of HSP90 ATPase homolog 2
• Synonyms
  • DKFZp564C236
  • Hch1
  • AHA1
• GenBank acession
  • AK092817
• Locus
  2p15
• Discovery year
  2003-04-04
• Entrez gene record
  130872
• Pubmed identfication
  • 12504007
• VEGA ID
  OTTHUMG00000129437

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products