CD16 / FcgammaRIII monoclonal antibody
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Catalog number1P-214-T025
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PricePlease ask
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Size25 assays
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Long nameMouse Monoclonal Antibody against Human CD16 / FcgammaRIII, Clone: MEM-154
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AntigenCD16
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Species reactivityHuman (Homo sapiens)
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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CloneMEM-154
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ApplicationsFC, IP, WB, FUNC
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ImmunogenHuman granulocytes
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Format conjugationPE
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SpecificityThe antibody MEM-154 reacts with an epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes._x000D_ HLDA V; WS Code M MA068_x000D_ HLDA V; WS Code NK NK51
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Research areaCD and Related Antigens (Human)
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Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storageMouse Monoclonal Antibody against Human CD16 / FcgammaRIII, Clone: MEM-154 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by affinity chromatography
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References*Gasdaska JR, Sherwood S, Regan JT, Dickey LF: An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab. Mol Immunol. 2012 Mar;50(3):134-41, *Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood. 1997 Aug 1;90(3):1109-14., *de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of_x000D_ human IgG by NK cell Fc gamma RIIIa. J Immunol. 1996 Apr 15;156(8):3948-55. _x000D_ , *Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. _x000D_ , *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)._x000D_ _x000D_
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolFCGR3A, FCGR3B
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Short nameAnti-CD16 / FcgammaRIII Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgG1
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Alternative nameMouse Monoclonal to CD16 / FcgammaRIII
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameFc gamma receptor IIIa
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
- Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
- Fc fragment of IgG receptor IIIa
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Synonyms
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GenBank acession
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Locus
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Discovery year1988-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameFc gamma receptor IIIb
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
- Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
- Fc fragment of IgG receptor IIIb
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-08-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data