Filters
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing

Mouse IL-7 PicoKine ELISA Kit

Mouse IL-7 PicoKine ELISA Kit is available 2 times from Boster labs

EK0780 | Mouse IL-7 PicoKine ELISA Kit size: 1x96 well plate | 678.85 USD

Loading

EK0780 | Mouse IL-7 PicoKine ELISA Kit size: 96wells/kit, with removable strips. | 625.41 USD

Loading
Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ELISA's application For quantitative measurement of mouse IL-7 in cell culture supernatant, serum and plasma EDTA .
More gene info Interleukin 7(IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7(IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17(T(H)17) cells in experimental autoimmune encephalomyelitis(EAE).2
Anti-coagulation EDTA
ELISA's sensitivity< 1pg/ml
Kit Components 10ng/tube
Uniprot Interleukin-7
Gen protein identity P10168
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
Immunogen Expression system for standard: E.coli; Immunogen sequence: E26-I154
Gene tested murine IL-7
ELISA standard curve Concentration(pg/ml) + 0 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.095 + 0.132 + 0.159 + 0.192 + 0.337 + 0.568 + 1.079 + 1.882
Gene name interleukin 7
ELISA kit description GENTAUR's mouse IL-7 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for IL-7 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, E26-I154) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-7 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse IL-7 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Minutes of TMB incubation 25-30
How the elisa works Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml mouse IL-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(EDTA) to the ELISA plate. Keep in mind that each mouse IL-7 standard solution and each sample be measured in duplicate.
Alternative IL 7;IL7 nirs variant 6;Interleukin 7;Interleukin7
Entrez nr. 16196
Applications ELISA
ELISA kit detection limits 15.6pg/ml to 1000pg/ml
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse IL-7 antibodies;1 ST0000-10;Lyophilized recombinant mouse IL-7 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse IL-7 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Antigen E.coli, E26-I154
Reactivity Mouse
Gene Name Il7
Assay range 15.6pg/ml-1000pg/ml
Sample Type cell culture supernates, serum and plasma(EDTA).
Target of this ELISA Natural and recombinant mouse IL-7
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);47.8;376;755;52.5;388;746 ELISA standard deviations 2.49;13.16;35.49;3.31;32.59;40.3 CV(%);5.2;3.5;4.7;6.3;8.4;5.4
Detector and capure antibodies A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus
Gene targetIL-7 PicoKine
Short name Mouse IL-7 PicoKine ELISA Kit
Technique ELISA kit, ELISA, Mouse
Host mouse
Species Mouse
Alternative name Mouse Interleukin-7 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Similar products
Mouse CD117 c kit PicoKine ELISA Kit Suppplier: boster
Price: 590.15 USD
Mouse CD117 c kit PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse Meteorin PicoKine ELISA Kit Suppplier: boster
Price: 590.15 USD
Mouse GAS6 PicoKine ELISA Kit Suppplier: boster
Price: 590.15 USD
Mouse SCF PicoKine ELISA Kit Suppplier: boster
Price: 590.15 USD
Mouse TNFR2 PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse PGLYRP1/Pgrps PicoKine ELISA Kit Suppplier: boster
Price: 590.15 USD
Mouse CD32 FCGR2b c PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse Eotaxin PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse RANK PicoKine ELISA Kit Suppplier: boster
Price: 648.15 USD
Mouse OPN PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse CXCL9 PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse ALK 1 ACVRL1 PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse beta IG H3 TGFBI PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse TNFRSF14 HVEM PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse Gp130 IL6ST PicoKine ELISA Kit Suppplier: MyBioSource
Price: 0.00 USD
Mouse FGF23 PicoKine ELISA Kit Suppplier: boster
Price: 590.15 USD
Ask
Ajax processing
Close
Mouse IL-7 PicoKine ELISA Kit - boster - Gentaur.com
Filters
+32-(0)1-658-90-45 lieven@gentaur.com
Contact us
Ajax processing
Close
Chat with gentaur.com employee