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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Cancer
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Reactivity
Human
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Residues
207-327 amino acids: MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 90% pure
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Concentration
0.5 mg/ml (lot specific)
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Form Buffer
Liquid in 20 mM Tris pH 8.0 containing 10% glycerol
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.
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Assay Information
Cell line: NIH-3T3 (Mus mosculus/ embryo) Maintenance Condition: RPMI 1640 containing 10% FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation Time: 24 hr (after sample treatment) Concentration Range: 100 pg/ml - 10 ug/ml Detection method: BrdU assay
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Additional Information
VEGF121 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugation
histidine