CD53, Mouse Monoclonal antibody-; Clone: MEM-53, FITC conj.
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Catalog numberACLX57F
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PricePlease ask
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Size100 tests
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Alternative name 1CD53, Mab anti-; Clone: MEM-53, FITC conj.
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Alternative name 2CD53, Mab antibody-; Clone: MEM-53, FITC conj.
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Alternative name 3CD53, Mab antibody-; Clone: MEM-53, FITC conj.
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Alternative name 4anti-CD53 Clone: MEM-53
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Alternative name 5CD53 Clone: MEM-53
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolIGLVIV-53, IGHV3-53, IGHVII-53-1, CD53, IGFBP3, RNU6-53P, SNORD8, RBBP4, IFT52, LMAN1
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Short nameCD53, Mouse Monoclonal antibody-; Clone: MEM-53, FITC conj.
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameCD53 molecule, Mouse monoclonal (antibody to-)-; clonality: MEM-53, fluorecein conj.
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD53 molecule, MOX44 and TSPAN25, CD53 and IDBG-101004 and ENSG00000143119 and 963, protein binding, Cell surfaces, Cd53 and IDBG-184902 and ENSMUSG00000040747 and 12508, CD53 and IDBG-635180 and ENSBTAG00000006466 and 505040
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (IV)-53 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (IV)-53
- immunoglobulin lambda variable (IV)-53 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-53
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-53-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-53-1
- immunoglobulin heavy variable (II)-53-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD53 molecule
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Synonyms gene
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Synonyms gene name
- CD53 antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tetraspanins
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinsulin like growth factor binding protein 3
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Synonyms gene name
- insulin-like growth factor binding protein 3
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-07-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Insulin like growth factor binding proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 53, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 53
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Locus
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Discovery year2011-08-08
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 8
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Synonyms gene
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Synonyms gene name
- RNA, U6C small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-01-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameRB binding protein 4, chromatin remodeling factor
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Synonyms gene name
- retinoblastoma-binding protein 4
- retinoblastoma binding protein 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-05-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EMSY complex
- SIN3 histone deacetylase complex subunits
- WD repeat domain containing
- NuRD complex subunits
- Polycomb repressive complex 2
- NURF complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintraflagellar transport 52
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Synonyms gene
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Synonyms gene name
- chromosome 20 open reading frame 9
- intraflagellar transport 52 homolog (Chlamydomonas)
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IFT-B1 complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelectin, mannose binding 1
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Synonyms gene
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Synonyms gene name
- coagulation factor V-factor VIII combined deficiency
- lectin, mannose-binding, 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-06-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data