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Long name
Guinea pig Antibody against goat IgG, FITC
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Synonym name
IgG goat Antibody
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Species reactivity
goat
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Tested applications recommended dilutions
ELISA
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Conjugation
FITC
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Antibody isotype
IgG
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
goat igg heavy chain (Native Protein)
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Immunogen species
goat
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Alias
IgG
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Background
The basic structure of IgG molecule is composed of four polypeptide chains, that is, from the same two small molecular weight peptides (referred to as light chain,Light chain, L chain, the relative molecular mass of about 25,000) and two identical large molecular weight peptides (called heavy chain, heavy chain, H Chain, relative molecular mass of about 55,000) composed. A natural two heavy chains of IgG molecules are always the same. The goat IgG antibodies can specifically recognize the heavy Chain.
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Clonality
Polyclonal antibody
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Shipping and storage
IgG Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
goat igg heavy chain
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans.
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French translation
anticorps
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Gene target
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Short name
Anti-IgG
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Technique
Antibody, anti, FITC, Goat, IgG, antibody to, antibodies against human proteins, antibodies for, Fluorescein, goats, IgGs
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Host
Guinea pig, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
FITC
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Species
Guinea Pig, Goats, Guinea Pigs, Pigs and Guinea pigs
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Alternative name
IgG Antibody,FITC conjugated
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Alternative technique
antibodies, caprine, fluorescine, immunoglobulins
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