anti-CYBB Antibody

• Catalog number
  25-944
• Price
  Please ask
• Size
  50 ug

• Gene info
  CYBB is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD).Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Gene names
  CYBB, CGD, GP91-1, GP91-PHOX, GP91PHOX, NOX2, AMCBX2, p91-PHOX
• Antibodie s label
  unlabelled serum
• Purity
  Antibody is purified by peptide affinity chromatography method.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYBB.
• Immuno application
  ELISA, Western Blot
• Antibody advice
  CYBB antibody can be used for detection of CYBB by ELISA at 1:1562500. CYBB antibody can be used for detection of CYBB by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Suspention pH azide
  Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
• Keep at
  For short periods of storage (days) store at 4 grades C. For longer periods of storage, store CYBB antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
• Works with
  Human, Mouse, Rat
• Omim nr
  6996021
• Ncbi nr
  NP_000388
• KDa
  63 kDa
• Vial with antibody
  Lyophilized
• Quantity per ml
  1 mg/ml
• NCBI GENE nr
  1536
• GENE symbol
  CYBB
• Antigene name
  cytochrome b-245, beta polypeptide
• Source
  Homo sapiens
• Protein nr
  P04839
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CYBB  
• Gene symbol
  CYBB
• Short name
  anti-CYBB Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toCYBB (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  cytochrome b-245, beta polypeptide, AMCBX2 and CGD and GP91-1 and GP91-PHOX and GP91PHOX and IMD34 and NOX2 and p91-PHOX, CYBB and IDBG-55775 and ENSG00000165168 and 1536, flavin adenine dinucleotide binding, Plasma membranes, Cybb and IDBG-131158 and ENSMUSG00000015340 and 13058, CYBB and IDBG-638147 and ENSBTAG00000019953 and 281112

Gene info

• Identity
  HGNC:2578
• Gene
  CYBB
• Long gene name
  cytochrome b-245 beta chain
• Synonyms gene
  • CGD
• Synonyms gene name
  • chronic granulomatous disease
  • cytochrome b-245, beta polypeptide
• Synonyms
  • GP91-PHOX
  • NOX2
• Synonyms name
  • NADPH oxidase 2
• GenBank acession
  • X04011
• Locus
  Xp21.1-p11.4
• Discovery year
  1986-01-01
• Entrez gene record
  1536
• VEGA ID
  OTTHUMG00000033175
• Locus Specific Databases
  • CYBBbase: Mutation registry for X-linked chronic granulomatous disease (previously known as X-CDGbase)
  • LRG_53

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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