ERK5 Antibody

• Catalog number
  F50463-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ERK5
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
• Notes
  Titration of the ERK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This ERK5 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q13164
• Purity
  Purified
• Description
  MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
• Immunogen
  A portion of amino acids 776-805 from the human protein was used as the immunogen for this ERK5 antibody.
• Storage
  Aliquot the ERK5 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ERK5  
• Gene symbol
  MAPK7
• Short name
  Anti-ERK5
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to ERK5
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6880
• Gene
  MAPK7
• Long gene name
  mitogen-activated protein kinase 7
• Synonyms gene
  • PRKM7
• Synonyms
  • BMK1
  • ERK5
• Synonyms name
  • BMK1 kinase
  • extracellular-signal-regulated kinase 5
• GenBank acession
  • U25278
• Locus
  17p11.2
• Discovery year
  1998-04-28
• Entrez gene record
  5598
• Pubmed identfication
  • 10072598
  • 7759517
• RefSeq identity
  • NM_139033
• Classification
  • Mitogen-activated protein kinases
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000059587

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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