Lactate Dehydrogenase Activity Colorimetric Assay Kit
Cell and tissue culture supernatants, urine, plasma and serum, other biological fluids etc.
The product Lactate Dehydrogenase Activity kit for Colorimetric Assayscontains the following reagents: LDH Assay Buffer, LDH Substrate Mix (lyophilized), NADH Standard (0.5 µmol; lyophilized), LDH Positive Control
Absorbance (450 nm)
The Lactate Dehydrogenase Activity kit for Colorimetric Assays is manufactured for direct detection of LDH that has a concentration range of 1 -100 mU/ml in samples.
The product Lactate Dehydrogenase Activity kit for Colorimetric Assaysensures simple, rapid and sensitive sample anlysis that take no more than 30 minutes.
Delivery and storage
The product Lactate Dehydrogenase Activity Colorimetric Assay Kit should be shipped on gel packages. Upon receipt, all reagents included in this kit should be kept at minus twenty degrees Celsius to retain their quality, activity and stability.
The product Lactate Dehydrogenase Activity Colorimetric Assay Kit can be used for one year if all reagents are stored according to the instructions.
The product Lactate Dehydrogenase Activity Colorimetric Assay Kit is intended to be used for research purposes only. It is not manufactured for application in diagnostics.
Lactate dehydrogenase (LDH) is an oxidoreductase (EC 126.96.36.199) present in a wide variety of organisms. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NADᶧ. When disease or injury or toxic material damages tissues, cells release LDH into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. In this colorimetric LDH quantification assay, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive. The kit can detect 1 -100 mU/ml of LDH directly in samples.
• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: The kit can detect 1 :100 mU/ml of LDH directly in samples.
• Simple procedure; takes ~ 30 minutes • Fast and convenient • Sensitive assays for measuring LDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit's other name
• LDH Assay Buffer • LDH Substrate Mix (lyophilized) • NADH Standard (0.5 µmol; lyophilized) • LDH Positive Control
Maximum time can be stored
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832