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Immunogen
Synthesized peptide derived from human OCT3 antibody.
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Antibody s target
Human 38261
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Antibody s full description
Rabbit Anti-Human OCT4 Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Octamer binding transcription factor 4 Antibody, MGC22487 Antibody, Oct 3 Antibody, Oct 4 Antibody, Oct-3 Antibody, Oct-4 Antibody, OCT3 Antibody, Oct4 Antibody, Octamer binding protein 3 Antibody, Octamer binding protein 4 Antibody, Octamer binding transcription factor 3 Antibody, Octamer-binding protein 3 Antibody, Octamer-binding protein 4 Antibody, Octamer-binding transcription factor 3 Antibody, OTF 3 Antibody, OTF 4 Antibody, OTF-3 Antibody, OTF3 Antibody, OTF4 Antibody, PO5F1_HUMAN Antibody, POU class 5 homeobox 1 Antibody, POU domain class 5 transcription factor 1 Antibody, POU domain transcription factor OCT4 Antibody, POU domain, class 5, transcription factor 1 Antibody, POU-type homeodomain-containing DNA-binding protein Antibody, POU5F1 Antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~39kDa, endogenous levels of total OCT3 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1304 was sufficient for detection of OCT4 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
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Scientific context
Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a protein that in humans is encoded by the POU5F1 gene. It is a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types.
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Bibliography
1. Takeda J. et al. (1992) Nucleic Acids Research 20 (17): 4613–20. 2. Niwa H. et al. (2000) Nature Genetics 24 (4): 372–6.
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Released date
6/Sep/2016
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NCBI number
NP_002692.2
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Gene number
5460
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Protein number
Q01860
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HeLa cell lysates showing detection of ~34kDa OCT4 protein using Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-OCT4 Polyclonal Antibody (SPC-1304) at 1:1000. Predicted/Observed Size: ~34kDa. Rabbit Anti-OCT4 Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1304) | Rabbit Anti-OCT4 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1304)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
