Syk Antibody (phospho-Y525/Y526)
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Catalog number
F48459-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Syk (phospho-Y525/Y526)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC, ELISA
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Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
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Notes
Titration of the phospho-Syk antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-Syk antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P43405
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Purity
Antigen affinity
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Description
Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.
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Immunogen
This phospho-Syk antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY525/526 of human Syk.
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Storage
Aliquot the phospho-Syk antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Anti-Syk (phospho-Y525/Y526)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Syk (phospho-Y525/Y526)
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Alternative technique
antibodies
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Alternative to gene target
spleen tyrosine kinase, p72-Syk, SYK and IDBG-75571 and ENSG00000165025 and 6850, transferase activity, Plasma membranes, Syk and IDBG-152695 and ENSMUSG00000021457 and 20963, SYK and IDBG-637026 and ENSBTAG00000004767 and 515515
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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