Human Recombinant IDH1

• Catalog number
  6379-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant IDH1
• Alternative_names
  Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD.
• Description
  Catalyzes the rate-limiting step in Citric acid cycle
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  >0.7 units/ml
• Results
  >0.7 units/ml
• Unit Definition
  One unit will convert 1.0 µmole of isocitrate to α-ketoglutarate per minute at pH7.5 at 25oC.
• Molecular Weight
  48.8 kDa (434 aa, 1-414 aa + NT His-Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl, 1 mM DTT and 0.1 mM PMSF
• Background Information
  Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  IDH1  
• Gene symbol
  IDH1, IDH1-AS1
• Short name
  Recombinant IDH1
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. IDH1
• Alternative technique
  rec

Gene info

• Identity
  HGNC:5382
• Gene
  IDH1
• Long gene name
  isocitrate dehydrogenase (NADP(+)) 1
• Synonyms gene name
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • isocitrate dehydrogenase 1 (NADP+)
  • isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  3417
• RefSeq identity
  • NM_005896
• Classification
  • Isocitrate dehydrogenases
• VEGA ID
  OTTHUMG00000132943
• Locus Specific Databases
  • LRG_610

Gene info

• Identity
  HGNC:40292
• Gene
  IDH1-AS1
• Long gene name
  IDH1 antisense RNA 1
• Synonyms gene name
  • IDH1 antisense RNA 1 (non-protein coding)
• GenBank acession
  • AI492612
  • BE465811
• Locus
  2q34
• Discovery year
  2012-02-24
• Entrez gene record
  100507475
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000154707

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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