Alternative name 1
CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mab anti-Rat, FITC; flow
Alternative name 2
CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mab antibody-Rat, FITC; flow
Alternative name 3
CD134, OX-40 Ligand, CD4 +'ve activated T-Cell Ag, 50kD, Clone: MRC OX-40, Mab antibody-Rattus norvegicustus norvegicus, FITC; flow
Alternative name 4
anti-CD134 OX-40 Ligand CD4 +'ve activated T-Cell Ag 50kD Clone: MRC OX-40 flow
Alternative name 5
CD134 OX-40 Ligand CD4 +'ve activated T-Cell Ag 50kD Clone: MRC OX-40 flow
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.For cells, cell lines and tissues in culture till half confluency.FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Mus musculus,Rattus norvegicus
CD134, OX-40 Ligand, CD4 +'ve activated T- Ag, 50kD, Clone: MRC OX-40, Mouse Monoclonal antibody- FITC;
Mouse monoclonal, Antibody, Mouse, FITC, Flow
CD134, OX-40 Ligand, CD4 molecule and've activated T-cellular Ag, 50kD, clonality: MRC OX-40, Mouse monoclonal (Antibody to)-Rat, fluorecein; flow