AKT2 (phospho-S474) polyclonal antibody
-
Catalog number
BS4720-100ul
-
Price
Please ask
-
Size
100ul
-
-
Alternative Name
RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ;
-
Immunogen
Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474.
-
Swiss Prot
P31751
-
Reactivity
Human,Mouse,Rat
-
Applications
WB IHC
-
Application_all
WB: 1:500~1:1000 IHC: 1:50~1:200
-
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
-
Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
-
Specificity
p-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.
-
Usage
Western blot (WB) analysis of p-AKT2(S474) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,15 minutes) whole cell lysate(40ug) Lane3:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug) Lane4:The Brain tissue lysate of Rat(40ug) Lane5:The Brain tissue lysate of Mouse(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug)
-
BiowMW
~ 60 kDa
-
Note
For research use only, not for use in diagnostic procedure.
-
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
AKT2
-
Short name
AKT2 (phospho-S474) polyclonal antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit
-
Alternative name
v-akt murine thymoma viral oncogene homolog 2 (phosphorilated-S474) polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
v-akt murine thymoma viral oncogene homolog 2, HIHGHH and PKBB and PKBBETA and PRKBB and RAC-BETA, AKT2 and IDBG-51270 and ENSG00000105221 and 208, transferase activity, nuclei, Akt2 and IDBG-161456 and ENSMUSG00000004056 and 11652, AKT2 and IDBG-637808 and ENSBTAG00000001400 and 534923
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
