IDO1, human recombinant
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Catalog numberP1096-10
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PricePlease ask
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Size10 ug
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SynonymsIndoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO
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Alternative_namesIndoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO
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DescriptionCatalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test methodMeasured by the hydrolysis of 1.0 pmole of L-Tryptophan to N-formyl-Lkynurenine per minute at pH 6.5 at 25C.
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Biological activitySpecific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-Lkynurenine per minute at pH 6.5 at 25C.
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Results>300 pmol/min/ug
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Unit DefinitionOne unit is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-Lkynurenine per minute at pH 6.5 at 25C.
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Molecular Weight47.7 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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Concentration0.5 mg/ml
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AppearanceLiquid
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Physical form descriptionIn 20mM Tris-HCl buffer (pH8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
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Background InformationIDO1 is an indoleamine 2,3-dioxygenase - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its pericellular catabolization of the essential amino acid tryptophan. Recombinant human IDO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MGSMAHAMEN SWTISKEYHI DEEVGFALPN PQENLPDFYN DWMFIAKHLP DLIESGQLRE RVEKLNMLSI DHLTDHKSQR LARLVLGCIT MAYVWGKGHG DVRKVLPRNI AVPYCQLSKK LELPPILVYA DCVLANWKKK DPNKPLTYEN MDVLFSFRDG DCSKGFFLVS LLVEIAAASA IKVIPTVFKA MQMQERDTLL KALLEIASCL EKALQVFHQI HDHVNPKAFF SVLRIYLSGW KGNPQLSDGL VYEGFWEDPK EFAGGSAGQS SVFQCFDVLL GIQQTAGGGH AAQFLQDMRR YMPPAHRNFL CSLESNPSVR EFVLSKGDAG LREAYDACVKALVSLRSYHL QIVTKYILIP ASQQPKENKT SEDPSKLEAK GTGGTDLMNF LKTVRSTTEK SLLKEG
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolIDO1
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Short nameIDO1, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameindoleamine 2,3-dioxygenase 1, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetindoleamine 2,3-dioxygenase 1, IDO and IDO-1 and INDO, IDO1 and IDBG-18750 and ENSG00000131203 and 3620, tryptophan 2, Cytoplasm, Ido1 and IDBG-142448 and ENSMUSG00000031551 and 15930, BT.19792 and IDBG-629572 and ENSBTAG00000020602 and 506281
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Gene info
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Identity
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Gene
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Long gene nameindoleamine 2,3-dioxygenase 1
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Synonyms gene
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Synonyms gene name
- indoleamine-pyrrole 2,3 dioxygenase
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GenBank acession
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Locus
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Discovery year1993-05-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data