HRP Western Blot Anti-IgG Antibody Kit
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Catalog number
OKRA00006
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Price
Please ask
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Size
one vial
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Recognized antigen
HRP Western Blot IgG Kit
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Product type
Antibody
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Gene name
HRP Western Blot IgG Kit
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Long name
Antibody against HRP Western Blot IgG Kit
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Storage
For shorter time periods the HRP Western Blot IgG Kit Antibodies may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds of the HRP Western Blot IgG Kit Antibodies which might cause change or loss of the desired properties.
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Tips
Small amounts of the HRP Western Blot IgG Kit antibody may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by aviva they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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French translation
anticorps
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Gene target
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Gene symbol
KIT, KITLG
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Short name
Anti-HRP Western Blot IgG Kit
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Technique
Western Blot, Antibody, anti-, anti, Blot, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, western Blots, IgGs
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Isotype
IgG, IgG
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Label
HRP
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Alternative name
HRP Western Blot IgG Kit Antibodies
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Alternative technique
antibodies, western, kits, immunoglobulins
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
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Identity
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Gene
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Long gene name
KIT ligand
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1991-06-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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