Human CellExp™ CD172a/ SIRP alpha, Extracellular Domain (ED), Human Recombinant

• Catalog number
  7506-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
• Alternative_names
  SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
• Description
  ≥95% pure, active, human cell expressed, human recombinant CD172A / SIRP - an immunoglobulin-like cell surface receptor for CD47
• Recombinant
  Yes
• Source
  HEK293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its binding ability in a functional ELISA.
• Biological activity
  Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 5 ug/ml (100 ul/well) can bind to biotinylated SIRPa/CD172a (ED) with a linear range of 0.05-2.5 ug/ml
• Binding Capacity
  Immobilized Human CD47 at 5 ug/ml (100 ul/well) can bind to biotinylated SIRPa/CD172a (ED) with a linear range of 0.05-2.5 ug/ml
• Molecular Weight
  This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of ~39 kDa (31–373 aa). Under both reducing and non-reducing conditions the protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in PBS, pH 7.4.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in distilled water to a concentration up to 2 mg/ml.
• Background Information
  Signal Regulatory Protein Alpha (SIRPa) or Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS-1) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. This protein supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and is found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein, which leads to the negative regulation of phagocytosis, mast cell activation and dendritic cell activation. Diseases associated with SIRPA include bladder neck obstruction and autoimmune hemolytic anemia.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  The CellExp™ CD172a/ SIRP alpha, Extracellular Domain (ED), Recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   CD172a   SIRP   alpha   Extracellular   Domain  
• Gene symbol
  SIRPB1, SIRPG, SIRPA
• Short name
  CellExp™ CD172a/ SIRP alpha, Extracellular Domain (ED), Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ CD172a/ SIRP a, Extracellular Domain (ED), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:15928
• Gene
  SIRPB1
• Long gene name
  signal regulatory protein beta 1
• Synonyms gene name
  • signal-regulatory protein beta 1
• Synonyms
  • SIRP-BETA-1
  • CD172b
• GenBank acession
  • Y10376
• Locus
  20p13
• Discovery year
  2001-06-21
• Entrez gene record
  10326
• Pubmed identfication
  • 9062191
  • 16339511
• RefSeq identity
  • NM_006065
• Classification
  • C1-set domain containing
  • Signal regulatory proteins
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000031676

Gene info

• Identity
  HGNC:15757
• Gene
  SIRPG
• Long gene name
  signal regulatory protein gamma
• Synonyms gene
  • SIRPB2
• Synonyms gene name
  • signal-regulatory protein beta 2
  • signal-regulatory protein gamma
• Synonyms
  • bA77C3.1
  • SIRP-B2
  • SIRPgamma
  • CD172g
• GenBank acession
  • AB042624
• Locus
  20p13
• Discovery year
  2001-09-17
• Entrez gene record
  55423
• Pubmed identfication
  • 11185750
  • 16339511
• RefSeq identity
  • NM_018556
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • C1-set domain containing
  • Signal regulatory proteins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000031680

Gene info

• Identity
  HGNC:9662
• Gene
  SIRPA
• Long gene name
  signal regulatory protein alpha
• Synonyms gene
  • PTPNS1
• Synonyms gene name
  • protein tyrosine phosphatase, non-receptor type substrate 1
  • signal-regulatory protein alpha
• Synonyms
  • SHPS1
  • SIRP
  • MYD-1
  • BIT
  • P84
  • SHPS-1
  • SIRPalpha
  • CD172a
  • SIRPalpha2
  • MFR
  • SIRP-ALPHA-1
• GenBank acession
  • D86043
• Locus
  20p13
• Discovery year
  1999-03-19
• Entrez gene record
  140885
• Pubmed identfication
  • 9070220
  • 9062191
  • 16339511
• RefSeq identity
  • NM_080792
• Classification
  • Ig-like cell adhesion molecule family
  • C1-set domain containing
  • Signal regulatory proteins
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000031682

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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