MLH1 Antibody, ALEXA FLUOR 594

  • Catalog number
    bs-0836R-A594
  • Price
    Please ask
  • Size
    100ul
  • Long name
    MLH1 Polyclonal Antibody, ALEXA FLUOR 594 Conjugated
  • Also known as
    Anti-MLH1 PAb ALEXA FLUOR 594
  • Category
    Conjugated Primary Antibodies
  • Conjugated with
    ALEXA FLUOR® 594
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    MLH1
  • Specificity
    This is a highly specific antibody against MLH1.
  • Modification
    Unmodified
  • Modification Site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Immunogen range
    520-570/756
  • Subcellular location
    Nucleus
  • Source
    This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MLH1
  • Gene ID Number
    4292
  • Swiss Prot
    P40692
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Cross reactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the antigen
    Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
  • Purification
    Purified by Protein A.
  • Storage conditions
    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
  • Excitation emission
    590nm/617nm
  • Synonyms
    FCC2; COCA2; HNPCC; hMLH1; HNPCC2; DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1
  • Properties
    For facs or microscopy Alexa 1 conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Conjugation
    Alexa Fluor, ALEXA FLUOR® 594
  • Conjugated
    Alexa conjugate 1
  • French translation
    anticorps
  • Gene target
    MLH1  
  • Gene symbol
    MLH1
  • Short name
    Anti-MLH1
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    ALEXA FLUOR 594
  • Alternative name
    MLH1 Polyclonal Antibody, ALEXA FLUOR 594
  • Alternative technique
    antibodies
  • Alternative to gene target
    mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, structure-specific DNA binding, nuclei, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee