Mouse Anti- Alginate Monoclonal IgG1 Kappa, Clone 4B10-1C5

  • Catalog number
    SMC-208D-BI
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    4B10-1C5
  • Immunogen
    Sodium Alginate conjugated to KLH
  • Antibody s full description
    Mouse Anti- Alginate Monoclonal IgG1 Kappa Antibody, Clone: 4B10-1C5: Biotin
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Alginic Acid Antibody, Algin Antibody, Sodium Alginate Antibody
  • Raised in
    Mouse
  • Antibody s target
    Alginate
  • Primary research fields
    Cell Signaling, Post-translational Modifications
  • Brandname
    None
  • Antibodies applications
    WB, IHC
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:500), IHC (1:200); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-208 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
  • Bibliography
    1. Zhai P., Chen X.B., and Schreyer D.J. (2013) Biofabrication. 5(1): 015009. 2. Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., and Elibol M. (2012) J Biosci Bioeng. 113(2):233-238.
  • Released date
    24-Jun-2014
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5 (SMC-208). Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-208) at 1:200. Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5 (SMC-208). Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-208) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa. Mouse Anti-Alginate Antibody [4B10-1C5] used in Immunohistochemistry (IHC) on Human Burned Skin (SMC-208) Mouse Anti-Alginate Antibody [4B10-1C5] used in Western Blot (WB) on ALL BSA-Alginate Conjugate (SMC-208)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse Anti- Alginate Monoclonal IgG1 Kappa, Clone 4B10-1C5
  • Technique
    Mouse, anti, antibody to, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1 Kappa, IgG1
  • Label
    Biotin
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to- Alginate monoclonal IgG1 Kappa, clonality 4B10-1C5
  • Alternative technique
    murine, antibodies
  • Clone name
    4B10-1C5
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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