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Synonyms
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
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Alternative_names
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
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Description
A protein required for iron delivery from transferrin to cells
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥92%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rhCD71/TFR at 10 ug/ml (100 ul/well) can bind human Transferrin. The EC50 of human Transferrin is 6.4 ng/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized rhCD71/TFR at 10 ug/ml (100 ul/well) can bind human Transferrin. The EC50 of human Transferrin is 6.4 ng/ml.
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Molecular Weight
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 76 kDa. The predicted N-terminus is Cys 89. DTT-reduced Protein migrates as 80-85 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukemia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TFRC
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Short name
CellExp™ CD71 / TFRC / TFR, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ CD71 / transferrin receptor (p90, CD71) / TFR, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
transferrin receptor (p90, CD71), CD71 and p90 and T9 and TFR and TFR1 and TR and TRFR, TFRC and IDBG-71016 and ENSG00000072274 and 102724492,7037, poly(A) RNA binding, Extracellular, Tfrc and IDBG-154429 and ENSMUSG00000022797 and 22042, TFRC and IDBG-633841 and ENSBTAG00000032719 and 100852305,504698
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