Mouse Anti-Mouse Synaptotagmin-10 Monoclonal IgG1, Clone S269-73

  • Catalog number
    SMC-423D-A655
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S269-73
  • Immunogen
    Fusion protein amino acids 248-335 (Cytoplasmic C2A domain) of mouse Synaptotagmin-10
  • Antibody s full description
    Mouse Anti-Mouse Synaptotagmin 10 Monoclonal IgG1 Antibody, Clone: S269-73: ATTO 655
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    SYT10 Antibody, SYT-10 Antibody, Synaptotagmin 10 Antibody, Synaptotagmin X Antibody, SytX Antibody, P65 Antibody, SVP65 Antibody, DKFZp781D2042 Antibody, SYT Antibody
  • Raised in
    Mouse
  • Antibody s target
    Synaptotagmin 10
  • Primary research fields
    Neuroscience, Cell Structure, Pre-Synaptic Markers
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~60kDa. Does not cross-react with Synaptotagmin-6 or Synaptotagmin-3 (or others).
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-423 was sufficient for detection of Synaptotagmin-10 in 20 µg of Synaptotagmin10 transiently-transfected COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasmic Vesicle
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
  • Bibliography
    1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815. 2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
  • Released date
    28-Feb-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_061273.1
  • Gene number
    54524
  • Protein number
    Q9R0N4
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-10 Monoclonal Antibody, Clone S269-73 (SMC-423). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-10 Monoclonal Antibody (SMC-423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Synaptotagmin-10 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 10 protein using Mouse Anti-Synaptotagmin 10 Monoclonal Antibody, Clone S269-73 (SMC-423). Primary Antibody: Mouse Anti-Synaptotagmin 10 Monoclonal Antibody (SMC-423) at 1:100, 1:250, 1:500, and 1:1000. Mouse Anti-Synaptotagmin-10 Antibody [S269-73] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-423) Mouse Anti-Synaptotagmin 10 Antibody [S269-73] used in Western Blot (WB) on Rat brain lysates (SMC-423)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    IGHV3-73, RNU6-73P, CPSF3, OR7E26P, USH1C
  • Short name
    Mouse Anti-Mouse Synaptotagmin-10 Monoclonal IgG1, Clone S269-73
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 655
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMouse Synaptotagmin-10 monoclonal IgG1, clonality S269-73
  • Alternative technique
    murine, antibodies
  • Clone name
    S269-73
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    RNA, U6 small nuclear 73, pseudogene
  • Synonyms gene
  • Synonyms gene name
    • RNA, U6 small nuclear 73
  • Locus
  • Discovery year
    2011-08-08
  • Entrez gene record
Gene info
  • Identity
  • Gene
  • Long gene name
    cleavage and polyadenylation specific factor 3
  • Synonyms gene name
    • cleavage and polyadenylation specific factor 3, 73kD subunit
    • cleavage and polyadenylation specific factor 3, 73kDa
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-31
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • MBL fold containing DNA/RNA interacting subfamily
    • Endoribonucleases
    • Cleavage and polyadenylation specific factor subunits
  • VEGA ID
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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