Mouse Anti-Mouse Synaptotagmin-10 Monoclonal IgG1, Clone S269-73
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Catalog numberSMC-423D-A655
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PricePlease ask
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Size100 µg
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CloneS269-73
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ImmunogenFusion protein amino acids 248-335 (Cytoplasmic C2A domain) of mouse Synaptotagmin-10
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Antibody s full descriptionMouse Anti-Mouse Synaptotagmin 10 Monoclonal IgG1 Antibody, Clone: S269-73: ATTO 655
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameSYT10 Antibody, SYT-10 Antibody, Synaptotagmin 10 Antibody, Synaptotagmin X Antibody, SytX Antibody, P65 Antibody, SVP65 Antibody, DKFZp781D2042 Antibody, SYT Antibody
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Raised inMouse
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Antibody s targetSynaptotagmin 10
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Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~60kDa. Does not cross-react with Synaptotagmin-6 or Synaptotagmin-3 (or others).
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-423 was sufficient for detection of Synaptotagmin-10 in 20 µg of Synaptotagmin10 transiently-transfected COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCytoplasmic Vesicle
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextSynaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
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Bibliography1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815. 2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
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Released date28-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_061273.1
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Gene number54524
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Protein numberQ9R0N4
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-10 Monoclonal Antibody, Clone S269-73 (SMC-423). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-10 Monoclonal Antibody (SMC-423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Synaptotagmin-10 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 10 protein using Mouse Anti-Synaptotagmin 10 Monoclonal Antibody, Clone S269-73 (SMC-423). Primary Antibody: Mouse Anti-Synaptotagmin 10 Monoclonal Antibody (SMC-423) at 1:100, 1:250, 1:500, and 1:1000. Mouse Anti-Synaptotagmin-10 Antibody [S269-73] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-423) Mouse Anti-Synaptotagmin 10 Antibody [S269-73] used in Western Blot (WB) on Rat brain lysates (SMC-423)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV3-73, RNU6-73P, CPSF3, OR7E26P, USH1C
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Short nameMouse Anti-Mouse Synaptotagmin-10 Monoclonal IgG1, Clone S269-73
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 655
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Synaptotagmin-10 monoclonal IgG1, clonality S269-73
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Alternative techniquemurine, antibodies
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Clone nameS269-73
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-73
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 73, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 73
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Locus
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Discovery year2011-08-08
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namecleavage and polyadenylation specific factor 3
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Synonyms gene name
- cleavage and polyadenylation specific factor 3, 73kD subunit
- cleavage and polyadenylation specific factor 3, 73kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MBL fold containing DNA/RNA interacting subfamily
- Endoribonucleases
- Cleavage and polyadenylation specific factor subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 7 subfamily E member 26 pseudogene
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 7, subfamily E, member 26 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-29
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Entrez gene record
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Pubmed identfication
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Classification
- Olfactory receptors, family 7
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUSH1 protein network component harmonin
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Synonyms gene
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Synonyms gene name
- deafness, autosomal recessive 18
- Usher syndrome 1C (autosomal recessive, severe)
- Usher syndrome 1C
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-06-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data