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Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse ERBB4-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 , The EC50 of biotinylated human NRG1 is 0.55-1.29 μg/ml.
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Synonym
c-erbB-4;Her4
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Sequence
Met1-Leu652
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Fusion tag
C-His
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Accession
Q99P91
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 95 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
71.5 kDa
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AP Mol Mass
113 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ERBB4
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Short name
Recombinant Mouse HER4 / ErbB4 Protein (His tag) 100µg
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
His
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse HER4 / v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Protein (histidine detection labelled) 100µg
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Alternative technique
rec, murine
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Alternative to gene target
v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian), ALS19 and HER4 and p180erbB4, ERBB4 and IDBG-79992 and ENSG00000178568 and 2066, transferase activity, nuclei, Erbb4 and IDBG-164078 and ENSMUSG00000062209 and 13869, ERBB4 and IDBG-643612 and ENSBTAG00000012647 and
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