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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser474 of human PAK4 (AA471-477)
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Antibody s target
Human PAK4 (pSer474)
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Antibody s full description
Rabbit Anti-Human PAK4 (pSer474) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
PAK 4 Antibody, PAK-4 Antibody, Pak4 Antibody, PAK4_HUMAN Antibody, Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs Antibody, Serine threonine kinase PAK 4 Antibody, Serine/threonine protein kinase PAK 4 Antibody, Serine/threonine protein kinase PAK4 Antibody, Serine/threonine-protein kinase PAK 4 Antibody, KIAA1142 Antibody, p21 activated kinase 4 Antibody, p21 Cdc42/Rac1-actiated kinase 4 Antibody, P21 protein (Cdc42/Rac) activated kinase 4 Antibody, p21(CDKN1A) activated kinase 4 Antibody, p21-activated kinase 4 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 64.072 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1044 was sufficient for detection of PAK4 (pSer474) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Highly expression in prostate, testis and colon.
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Scientific context
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_005875
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Gene number
10298
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Protein number
O96013
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
