EpiQuik Hot Taq 2x PCR Mix

  • Catalog number
    R12019-1
  • Price
    Please ask
  • Size
    1 mL
  • Storage
    Our specialists from Gentaur/Genprice advise to follow carefully the instructions for use and store the EpiQuik Hot Taq 2x PCR Mix in refrigerator and according to the label upon arrival.
  • Tips
    Manufactured in United States. Designed for Research Use Only, not for human or animal consumption.
  • Test
    Chromatin isolation for histone extracts like H2A, H2B, H3, and H4 extraction for SDS-PAGE electrophoresis. Histones act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Hot start taq polymerases can be supplied as mix with dNTPs or Mg+ free Polymerases. The units are redifined for reactions but you can delute more this Hot start TAQ polymerase. Storage at -25 º C.
  • Gene target
    Hot   Taq  
  • Short name
    EpiQuik Hot Taq 2x PCR
  • Technique
    Epiquik, PCR, epiquik, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Epigen TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
  • Alternative name
    EpiQuik Hot Taq 2x PCR test kit Mix
  • Alternative technique
    epigenetic, dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
  • Tree numbers
    • E05.393.620
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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