AKT1 Antibody (pThr308): FITC

• Catalog number
  SPC-1341D-FITC
• Price
  Please ask
• Size
  100 µl

• Target
  AKT1 (pThr308)
• Conjugate
  FITC
• Research Area s
  Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Cancer, Cancer Metabolism, Cancer, Cell Cycle, PI3k/AKT Pathway
• Alternative Name s
  RAC-PK-alpha Antibody, Protein kinase B alpha Antibody, RAC Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, RAC Alpha Antibody, AKT Antibody, EC:2.7.11.1 Antibody, AKT 1 Antibody, PRKBA Antibody, AKT1_HUMAN Antibody, AKT1 Antibody, PKB Antibody, PKB-ALPHA Antibody, MGC99656 Antibody, PKB alpha Antibody, RAC-alpha serine/threonine-protein kinase Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human AKT1 around the phosphorylation site of threonine 308 (pThr308).
• Immunogen Species
  Human
• Accession Number
  NP_001014431.1
• Gene ID
  207
• Swiss Prot
  P31749
• Applications
  WB, IHC, ICC/IF
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:500); IHC (1:25-1:50); ICC/IF (1:50-100); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
• Specificity
  Detects endogenous levels of AKT1 only when phosphorylated at threonine 308 (pThr308).
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Certificate of Analysis
  A 1:500 dilution of SPC-1341 was sufficient for detection of AKT1 (pThr308) in 10 µg of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
• Cellular Localization
  Nucleus, Plasma Membrane, Cell Membrane, Cytoplasm, Cytoskeleton, Ciliary Basal Body, Microtubule, Spindle, Cytosol, Mitochondrion, Nucleoplasm, Cell-Cell Junction, Postsynapse, Protein Complex, Vesicle
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  AKT1   pThr308  
• Gene symbol
  AKT1
• Short name
  AKT1 Antibody (pThr308): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Isotype
  IgG
• Label
  FITC
• Alternative name
  v-akt murine thymoma viral oncogene homolog 1 (antibody to-) (pThr308): fluorecein
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and

Gene info

• Identity
  HGNC:391
• Gene
  AKT1
• Long gene name
  AKT serine/threonine kinase 1
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 1
• Synonyms
  • RAC
  • PKB
  • PRKBA
  • AKT
  • RAC-alpha
• Synonyms name
  • protein kinase B
• GenBank acession
  • M63167
• Locus
  14q32.33
• Discovery year
  1986-01-01
• Entrez gene record
  207
• Pubmed identfication
  • 21214427
• RefSeq identity
  • NM_005163
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000170795
• Locus Specific Databases
  • LRG_721

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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