BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible

  • Catalog number
    K819-250
  • Price
    Please ask
  • Size
    250 assays
  • Description
    Only kit on the market that is compatible with higher conc. of reducing agents TCEP (20 mM), DTT (10 mM), β-ME (35 mM)
  • Summary
    • Detection method- Absorbance (562 nm) • Reducing agent compatible
  • Detection Method
    Absorbance (562 nm)
  • Applications
    Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
  • Sample Type
    pure proteins, cell/tissue extracts or lysates
  • Features Benefits
    • Simple & convenient. • Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM). • The assay is linear over a wide range of protein concentration (25-2000 μg/ml).
  • Storage Conditions
    RT
  • Shipping Conditions
    RT
  • Shelf life
    12 months
  • Background
    BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This kit is adapted from BioVision’s BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determining protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over the widest range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene target
    BCA   Protein   Kit   Test   Reducing   Agent   Compatible  
  • Gene symbol
    CXCL13, BLNK
  • Short name
    BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible
  • Technique
    Assay, tube, assays, Biovision tubes
  • Host
    Assay
  • Alternative name
    Bicinchoninic acid Protein test reagent (Test Tube) - Reducing substance Compatible
  • Alternative technique
    arrays, tests, tubes, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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