Carboxyl Fluorescent Particles, Aqua Green, Flow Cytometry Grade, 0.1-0.3µm, ~2.9x10^10/mL, 10mL
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Catalog number
CFP01-0279-10
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Price
Please ask
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Size
10 mL
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Description
Carboxyl Fluorescent Particles, Aqua Green, Flow Cytometry Grade, 0.1-0.3µm, ~2.9x10^10/mL are a used in cell biology assays such as cell counting, cell separation, flow cytometry (FC), calibration of flow cytometer machines and so on. Guaranteed to meet the size specification and produced from high quality materials, these microparticles will be a handy tool in your lab work.
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Notes
For research use only.
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Additional description
FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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Gene target
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Gene symbol
IGKV1OR2-0, SNORD112, H1-0, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1
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Short name
Carboxyl Particles, Aqua , Grade, 1- 3µm, ~2 9x10^10/ , 10mL
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Technique
Flow Cytometry, Flow
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Label
fluorescent
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Alternative name
Carboxyl Fluorescent particles, Aqua Green, Flow Cytometry Grade, 0.1-0.3µm, ~2.9x10^10/milliliter, 10mL
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Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
H1.0 linker histone
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Synonyms gene
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Synonyms gene name
- H1 histone family, member 0
- H1 histone family member 0
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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