-
Antibody Target
SHH
-
Target Alternative Name
Sonic hedgehog protein, HHG-1, SHH
-
Antibody Type
Polyclonal
-
Species Reactivities
Human, Mouse
-
Immunogen Sequence
mouse recombinant SHH (Cat. No. 4020)
-
Accession
Q62226
-
Gene Id
20423
-
Appearance
Colorless liquid
-
Form
Liquid
-
Concentration
0.5 mg/ml
-
Formulation
100 µg (0.5 mg/ml) of antibody in PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin®, and 50 % glycerol.
-
Purification
Affinity purified rabbit IgG
-
Application
Western blot
-
Positive Control
Western blot
-
Application And Usages
Western blot: 1-4 µg/ml
-
Description
Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO.
-
Storage Condition
-20°C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans.
-
Handling
The antibody solution should be gently mixed before use.
-
Western Blot Verified
Verified
-
Immunocytochemistry Verified
Not Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Not Verified
-
ELISA Verified
Not Verified
-
ChIP Verified
Not Verified
-
Dot Blot Verified
Not Verified
-
Flow Cytometry Verified
Not Verified
-
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
SHH
-
Short name
SHH Polyclonal Antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Isotype
Rabbit IgG
-
Alternative name
sonic hedgehog polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
sonic hedgehog, HHG1 and HLP3 and HPE3 and MCOPCB5 and SMMCI and TPT and TPTPS, SHH and IDBG-50464 and ENSG00000164690 and 6469, laminin-1 binding, nuclei, Shh and IDBG-144309 and ENSMUSG00000002633 and 20423, SHH and IDBG-638249 and ENSBTAG00000024552 and
-
