Paf1 polyclonal antibody

  • Catalog number
    6831-25
  • Price
    Please ask
  • Size
    25 μg
  • Antibody Target
    Paf1
  • Target Alternative Name
    PD2
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human.
  • Immunogen Sequence
    Polyclonal antibody raised in rabbit against human Paf1 (Paf1, RNA polymerase II associated factor, homolog), using the full length recombinant protein.
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In PBS with 0.05% sodium azide and 0.05% ProClin 300.
  • Purification
    Protein G purified
  • Application
    Western blot, ChIP.
  • Positive Control
    Western blot: HeLa cells, ChIP.
  • Application And Usages
    WB: 1:1000, ChIP: 1-5 µg/ChIP.
  • Description
    PAF is a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9, which was first purified from yeast. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. The human PAF (hPAF) complex shares four subunits with yPAF (hCtr9, hPaf1, hLeo1 and Cdc73), but contains an additional higher eukaryotic-specific subunit, hSki8. In addition to coordinating events during transcription (initiation, promoter clearance and elongation); PAF also coordinates events in RNA quality control.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • French translation
    anticorps
  • Gene target
    Paf1  
  • Gene symbol
    PAF1, LEO1, RTF1
  • Short name
    Paf1 polyclonal antibody
  • Technique
    Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Isotype
    Rabbit IgG
  • Alternative name
    Paf1 polyclonal (antibody to-)
  • Alternative technique
    polyclonals, antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    LEO1 homolog, Paf1/RNA polymerase II complex component
  • Synonyms gene name
    • Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
  • GenBank acession
  • Locus
  • Discovery year
    2005-07-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Paf1/RNA polymerase II complex
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    RTF1 homolog, Paf1/RNA polymerase II complex component
  • Synonyms gene
  • Synonyms gene name
    • KIAA0252
    • Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
  • GenBank acession
  • Locus
  • Discovery year
    2004-03-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Paf1/RNA polymerase II complex
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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