Paf1 polyclonal antibody
-
Catalog number6831-25
-
PricePlease ask
-
Size25 μg
-
-
Antibody TargetPaf1
-
Target Alternative NamePD2
-
Antibody TypePolyclonal
-
Species ReactivitiesHuman.
-
Immunogen SequencePolyclonal antibody raised in rabbit against human Paf1 (Paf1, RNA polymerase II associated factor, homolog), using the full length recombinant protein.
-
AppearanceColorless liquid
-
FormLiquid
-
FormulationIn PBS with 0.05% sodium azide and 0.05% ProClin 300.
-
PurificationProtein G purified
-
ApplicationWestern blot, ChIP.
-
Positive ControlWestern blot: HeLa cells, ChIP.
-
Application And UsagesWB: 1:1000, ChIP: 1-5 µg/ChIP.
-
DescriptionPAF is a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9, which was first purified from yeast. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. The human PAF (hPAF) complex shares four subunits with yPAF (hCtr9, hPaf1, hLeo1 and Cdc73), but contains an additional higher eukaryotic-specific subunit, hSki8. In addition to coordinating events during transcription (initiation, promoter clearance and elongation); PAF also coordinates events in RNA quality control.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedNot Verified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Dot Blot VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translationanticorps
-
Gene target
-
Gene symbolPAF1, LEO1, RTF1
-
Short namePaf1 polyclonal antibody
-
TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
IsotypeRabbit IgG
-
Alternative namePaf1 polyclonal (antibody to-)
-
Alternative techniquepolyclonals, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namePAF1 homolog, Paf1/RNA polymerase II complex component
-
Synonyms gene name
- Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
-
Synonyms
-
Locus
-
Discovery year2005-07-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Paf1/RNA polymerase II complex
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameLEO1 homolog, Paf1/RNA polymerase II complex component
-
Synonyms gene name
- Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
-
GenBank acession
-
Locus
-
Discovery year2005-07-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Paf1/RNA polymerase II complex
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRTF1 homolog, Paf1/RNA polymerase II complex component
-
Synonyms gene
-
Synonyms gene name
- KIAA0252
- Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
-
GenBank acession
-
Locus
-
Discovery year2004-03-03
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Paf1/RNA polymerase II complex
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data