Synonyms Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF.
Alternates names Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase.
Taglines A protein which triggesr an acute immune response
NCBI Gene ID #. 4282
NCBI Gene Symbol MIF
Gene Source Human
Accession # P14174
Source E. coli
Activity (Specifications/test method) N/A
Biological activity Human MIF biological activity was measured in an agarose micro droplet assay using human U937 cells as targets. The optimal range of activity found for human MIF in this assay was 0.5-1µg/ml.
Molecular Weight 12.5 kDa
Storage Temp. -20°C
Shipping Gel pack
Shelf Life 1 year
Appearance Lyophilized protein
Physical form description MIF-Protein was lyophilized from 10 mM sodium phosphate buffer pH-7.5.
Reconstitution Instructions Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Short name MIF, recombinant
Alternative name macrophage migration inhibitory factor (glycosylation-inhibiting factor), H. sapiens recombinant
Alternative technique rec
Alternative to gene target macrophage migration inhibitory factor (glycosylation-inhibiting factor), GIF and GLIF and MMIF, MIF and IDBG-401910 and ENSG00000240972 and 4282, phenylpyruvate tautomerase activity, Extracellular, Mif and IDBG-163956 and ENSMUSG00000033307 and 17319, MIF and IDBG-637094 and ENSBTAG00000007375 and 280858