Protein A/G
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Catalog number6502-25
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PricePlease ask
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Size25 mg
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SynonymsProtein A/G
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Alternative_namesProtein A/G
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DescriptionGenetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Binding CapacityUnder optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
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Molecular Weight59.7 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized powder
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Reconstitution InstructionsReconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
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Background InformationProtein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Gene target
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Gene symbolGPRC6A, GPR162, PURG
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Short nameProtein A/G
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Alternative nameProtein A/G
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Gene info
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Identity
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Gene
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Long gene nameG protein-coupled receptor class C group 6 member A
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Synonyms gene name
- G protein-coupled receptor, family C, group 6, member A
- G protein-coupled receptor, class C, group 6, member A
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-26
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Entrez gene record
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Classification
- Calcium sensing receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameG protein-coupled receptor 162
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- G protein-coupled receptors, Class A orphans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepurine rich element binding protein G
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-02-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID