Protein A/G

  • Catalog number
    6502-25
  • Price
    Please ask
  • Size
    25 mg
  • Synonyms
    Protein A/G
  • Alternative_names
    Protein A/G
  • Description
    Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Purity
    ≥98%
  • Binding Capacity
    Under optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
  • Molecular Weight
    59.7 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized powder
  • Reconstitution Instructions
    Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
  • Background Information
    Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene target
    Protein   A/G  
  • Gene symbol
    GPRC6A, GPR162, PURG
  • Short name
    Protein A/G
  • Alternative name
    Protein A/G
Gene info
  • Identity
  • Gene
  • Long gene name
    G protein-coupled receptor class C group 6 member A
  • Synonyms gene name
    • G protein-coupled receptor, family C, group 6, member A
    • G protein-coupled receptor, class C, group 6, member A
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2002-04-26
  • Entrez gene record
  • Classification
    • Calcium sensing receptors
  • VEGA ID
Gene info
Gene info
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