M13/fd/F1 Filamentous Phages antibody (Biotin)

• Catalog number
  GEN532875
• Price
  Please ask
• Size
  50ug

• Also known as
  M13/fd/F1 Filamentous Phages (Biotin)
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2b
• Clone
  B62-FE2
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  M13/fd/F1 Filamentous Phages antibody (Biotin) was purified by Protein A affinity chromatography
• Form Appearance
  Supplied in lyophilized form. Reconstitute in 1 ml distilled water. Final solution contains 0.09 % NaN3, 0.5% BSA in PBS buffer, pH 7.4
• Concentration
  N/A
• Storage and shipping
  For shorter periods of time keep the antibody at +4 degrees Celsius. Long term storage should be done after aliquoting and at -20°C. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies, thus reducing their activity.
• Tested applications
  ELISA (EIA), Western Blot (WB)
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Gene
  M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
• French translation
  anticorps

• Gene target
  M13/fd/F1   Filamentous   Phages  
• Short name
  M13/fd/F1 Filamentous Phages antibody ( )
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  Anti-M13/fd/F1 Filamentous Phages antibody (Biotin)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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