Reality Reverse Transcriptase cDNA Synthesis Kit
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Catalog number
MI-CDNA
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Price
Please ask
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Size
100 rxn
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Description
this 1 cDNA is double stranded complementary DNA derived by Molecular Innovations from mRNA with very high fidelity reverse transcription using a pfu or pfx mix, Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Short name
Reality Reverse Transcriptase cDNA Synthesis Kit
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Technique
reverse, cDNA, cDNAs
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Alternative name
Reality Reverse Transcriptase complementary Desoxyribonucleic acid Synthesis reagent
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Alternative technique
transctription, pcr, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cdna
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MeSH Data
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Name
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Concept
Scope note:
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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