Recombinant Human Prostate-Specific Antigen//PSA/KLK3 (Ala18-Pro261, C-6His)
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Catalog number
C365-1000
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Price
Please ask
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Size
1 mg
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Description
Recombinant Human Kallikrein 3 is produced by our Mammalian expression system and the target gene encoding Ala18-Pro261 is expressed with a 6His tag at the C-terminus.
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Species reactivity
Human
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Origin
Human Cells
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Peptide sequence
APLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANPVDHHHHHH
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Estimated molecular weight
27,88 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Ambient/Room Temperature
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Package form
Lyophilized from a 0.2 µm filtered solution of 20mM MES, 150mM NaCl, 2mM CaCl2, pH 6.0.
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Storage conditions
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P07288
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Additional description
Antigens are peptides or recombinant or native dependent on the production method.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
KLK3, KLKB1, NPEPPS, PSAT1
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Short name
Recombinant Prostate-Specific Antigen//PSA/KLK3 (Ala18-Pro261, C-6His)
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Technique
Recombinant, antigen, antigenes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human Kallikrein 3/KLK3/PSA(Ala18-Pro261,C-6His)
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Alternative technique
rec, antigenes
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Tissue
prostate
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Gene info
Gene info
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Identity
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Gene
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Long gene name
kallikrein B1
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Synonyms gene
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Synonyms gene name
- kallikrein B, plasma (Fletcher factor) 1
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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