Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

• Catalog number
  K745-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay, HTS
• Summary
  • Detection method- Fluorescence (Ex/Em = 485/525 nm) • Species reactivity- Mammalian
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
• Features Benefits
  • Simple procedure • Fast and convenient • Sensitive assays for measuring MPO as low as 0.5 μU per well
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In BioVision’s MPO Assay Kit, MPO catalyzes the production of NaClO from H₂O₂ and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 μU per well.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Myeloperoxidase   MPO   Fluorometric   Activity   Kit  
• Gene symbol
  MPO
• Short name
  Myeloperoxidase (MPO) Fluorometric Activity Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Myeloperoxidase (myeloperoxidase) fluorescence Activity test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:7218
• Gene
  MPO
• Long gene name
  myeloperoxidase
• Locus
  17q22
• Discovery year
  2001-06-22
• Entrez gene record
  4353
• VEGA ID
  OTTHUMG00000178922
• Locus Specific Databases
  • MPObase: Mutation registry for Myeloperoxidase deficiency
  • LRG_84

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