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Human Glycogen debranching enzyme ELISA Kit

Human Glycogen debranching enzyme ELISA Kit is available 1 time from Sab elisa kit labs

EK3720 | Human Glycogen debranching enzyme ELISA Kit size: 96-wells plate (breakable strips) | 766.53 USD

Catalog number EK3720
Price 766.53 USD
Size 96-wells plate (breakable strips)
1. Gene info
Identity 321
Gene AGL
Long gene name amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Synonyms gene name
  • amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Synonyms name
  • glycogen debranching enzyme
  • glycogen storage disease type III
GenBank acession
  • BC078663
Locus 1p21.2
Discovery year 1992-07-29
Entrez gene record 178
Pubmed identfication
  • 1505983
RefSeq identity
  • NM_000028
Havana BLAST/BLAT OTTHUMG00000010803
MeSH Data
Name Enzyme-Linked Immunosorbent Assay
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
Product images
    #Human#.jpg
Long name ELISA test kit for Human Glycogen debranching enzyme
Synonym name Homo sapiens Glycogen debranching enzyme ELISA Kit
General description This is a ready to use kit for performing ELISA tests for detection of Glycogen debranching enzyme in samples from Human
Antigen Glycogen debranching enzyme
Species reactivity Human
Sample types Our Glycogen debranching enzyme ELISA kit has been proven to work and successfully detect Glycogen debranching enzyme in Human
Kit contents ELISA test kit for Human Glycogen debranching enzymeAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
Detection wavelengt 450 nm
Assay procedure 1-4h
Summary of the prodecure 1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
Applications Enzyme Linked Immunosorbent Assay
Shipping, storage, shelf life The Glycogen debranching enzyme ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human Glycogen debranching enzyme ELISA Kit is 6 months after delivery.
Tips Due to transportation and handling small amounts of the liquid components of the Homo sapiens Glycogen debranching enzyme ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Gene targetGlycogen debranching
Short name Glycogen debranching enzyme ELISA Kit
Technique ELISA kit, Enzyme, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative name H. sapiens Glycogen debranching enzyme Enzyme-linked immunosorbent assay reagent
Alternative technique enzymes
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Human Glycogen debranching enzyme ELISA Kit size: 96-wells p
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EU:+32-(0)1-658-90-45 US:+1-(408)780-0908 [email protected]
  • Glycogen
  • debranching
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