Protein A/G/L Sepharose

  • Catalog number
    6541-25
  • Price
    Please ask
  • Size
    25ml
  • Description
    Reusable Protein A/G/L conjugated sepharose beads with ≥10 mg/ml binding capacity & high flow rate for antibody purification from multiple sources. Protein A/G/L-Sepharose is prepared by covalently coupling recombinant Protein A/G/L (Cat.# 6540; containing five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding regions of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding) to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet gel.
  • Product Highlights
    • CONTENTS: Supplied as a 50% slurry in 20% Ethanol/H₂O; >5 mg Protein A/G/L per ml of sepharose beads. • FEATURES: Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. The binding capacity is greater than 10 mg/ml of gel; High flow rate; Low falling off of rProtein A/G/L; pH stability 2-10. • APPLICATIONS: Purification of monoclonal and polyclonal antibodies, Immunoprecipitation.
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Gene target
  • Short name
    Protein A/G/L Sepharose
  • Alternative name
    Protein A/G/L Sepharose
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