IgG2a Isotype Control Monoclonal Antibody [C1.18], FITC Conjugated

• Catalog number
  A24004-1
• Price
  Please ask
• Size
  0,2 mg

• Target antigen
  IgG2a Isotype Control
• Clone
  C1.18
• Conjugation additive
  FITC Conjugated
• Product type
  Antibody
• Clonality
  monoclonal antibody
• Long name
  IgG2a Isotype Control Monoclonal Antibodies [C1.18], FITC Conjugated
• Description
  IgG2a Isotype Control. Monoclonal antibodies ensure a highly specific binding to a certain epitope of the antigen and are much more LOT-to-LOT consistent than polyclonal antibodies.
• Storage conditions
  For short term storage the IgG2a Isotype Control Monoclonal Antibody [C1.18], FITC Conjugated can be kept refrigerated at +4 degrees Celsius. For long term storage keep frozen at -20 degrees Celsius. Be sure to refer to the technical sheet for any additional specifications and requirements for proper storing of the antibodies.
• Tips
  IgG2a Isotype Control MAb [C1.18], FITC Conjugated should not be exposed to cycles of freezing and thawing as they may denaturate the peptide chains/bonds in the antibodies and reduce/change the activity of the antibody. Prior to use briefly centrifuge the vial to collect its content on the bottom.
• Properties
  If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Epigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Epigen FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Additional description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Conjugation
  Anti-FITC Antibody
• Group
  isotypes
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  IgG2a   Monoclonal   [C1   18]   Conjugated  
• Short name
  Anti-IgG2a Isotype Control
• Technique
  Antibody, Control, Isotype, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, controls, Fluorescein, Isotypes and controls, Monoclonals or monoclonal antibodies
• Isotype
  IgG2a
• Label
  FITC
• Alternative name
  IgG2a Isotype Control MAb [C1.18], FITC Conjugated
• Alternative technique
  antibodies, control, controls, fluorescine
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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